Central Research Laboratory

The Central Research Laboratory has been operating since October 1989 according to the Order of the Ministry of Health of Ukraine No. 177, dated August 21, 1989.

The laboratory is a research department of an innovative educational institution, where development has become an objective necessity, and combining educational processes with innovative and research work is a usual activity of the academic staff, who absorb new methods of work and implement innovative technologies into their professional activities.

The research laboratory performs experimental work in situ by combining the educational process with the research work, which is necessarily planned (projected), managed and controlled.

The laboratory contributes to the development of scientific thinking and is an experimental platform for training of high skilled academic staff of the university: teachers and researchers who are experimenters as well.

The Head of the Central Research Laboratory is Senior Researcher, Associate Professor Natalia Yevhenivna Lisnychuk.

The facilities of the Central Research Laboratory are a special purpose premises for carrying out the studies declared and confirmed by the Certificates of Attestation (Certificate of Attestation No. 001488, issued on November 03, 2003 valid until November 03, 2007; Certificate of Attestation No. 000478, issued on December 17, 2007, valid until December 16, 2012; Certificate of Attestation No. 053/13, issued on March 04, 2013, valid until March 03, 2018).

The design of the Central Research Laboratory has been carried out following all requirements regarding the research laboratories according to the principles and rules of Good Laboratory Practice (GLP):

• the laboratory has the appropriate sizes, design and location that meet the requirements for the experiments and facilitate minimizing the deviations that may adversely influence the reliability of the results;
• the design of the experimental laboratory ensures adequate degree of distribution of various activities to warrant that each type of research is conducted properly;
• in the Central Research Laboratory there is a sufficient number of rooms and a separate area for keeping test systems (laboratory animals) for their adaptation as well as culling of off-test animals;
• there are isolated premises for storing consumables (chemical reagents), classified as hazardous, for their safe storage.

Central Research Laboratory Structure:

1. Laboratory for Experimental Simulation.
2. Laboratory for Immunological Investigations.
3. Laboratory for Biochemical Investigations.
4. Laboratory for Histological and Electron Microscopic Studies.

The laboratory staff involves high skilled experts that ensures a proper distribution of various activities to warrant that each type of research is properly conducted according to the principles and rules of GLP.

The scientific researches of the Central Research Laboratory include study of patterns of pathological and adaptation-compensatory processes in the body of experimental animals in cases of osteoporosis, cardiovascular, respiratory and digestive disorders, oncological diseases, experimental justification of new methods of their prevention and correction.

The laboratory staff has developed and implemented into practice of experimental studies a model of experimental colitis in different animals, a model of acute and chronic experimental pancreatitis, a model of chronic cholecystitis, a model of acute circumscribed purulent staphylococcal endometritis in cavy, a model of hepatic impairment, a model of LPS-induced chronic endotoxemia, a model of chronic neoplastic endogenous intoxication with induction of colon adenocarcinoma development.

The results of the experimental studies allowed developing new methods of detoxification and rehabilitation using up-to-date sorption agents: herbal sorption factors Carnitine chloride and Algigel, polyfunctional carbon enterosorbents of the fourth generation HSGD (Hemosorbent granulated deligandizing) and Carboline.

All new ways of experimental simulation of pathologic processes as well as the methods of their correction developed and implemented in the health care have been secured by the title of protection of Ukraine.
The algorithm of choosing the terms and volume of surgical intervention in the patients with duodenal peptic ulcer bleeding according to immunomorphological assessment of the factors of periulcerous region defence, ulcerative destruction, probability of bleeding relapse as well as scale of surgery and anaesthesia risk in different age groups in cases of comorbidity.
Endoscopic and immunomorphological monitoring of the activity, dynamics and prognosis of duodenal ulcer complicated by bleeding, which at present is widely used in clinical departments of surgery, is suggested.
A new method of computer morphometric analysis of histological specimens has been implemented into the practice of the Central Research Laboratory using a system of visual image analysis of micropreparations by means of Vision Color CCD video camera and Inter Video Win DVR UTHSCSA Image Tool program.

Constant advancement of the Central Research Laboratory with innovative scientific equipment contributes to the expansion and updating of the investigations being carried out there.

Tissue grinding by means of up-to-date magnetic homogenizer

Silent Crusher S with a rotor speed of up to 75.000 rpm allowed obtaining the homogenate of the investigated subjects with a particle size of up to 0.4 mm. Such prototypes are subjected to the study of the dynamics of necrosis and apoptosis of cellular structures by means of flow cytofluorometry.

In 2010-2014 the researchers of the Central Research Laboratory have completed 227 theses:

• 18 doctorate thesis;
• 209 PhD theses;
• 4 master’s theses;
• 7 state financed academic research works;
• 9 contract-based researches.

In 2010-2014, according to the materials of academic research works performed in the Central Research Laboratory the following works have been published:

• 47 articles and conference abstracts (3 articles and 14 conference abstracts in the world literature);
• 7 patents.

The innovation of “Complex of sorption and magnetic-laser therapy for rehabilitation of the patients with manifestations of reactive enteritis combined with disorders of organs of the pancreatohepatobiliary region”, which was developed by the researchers of the Central Research Laboratory, is registered in the Register of Sectoral Innovations No. 32/33, year 2010, with No. 50/32/10.
4 innovations in the health care have been published:
Notice No. 112-2014 “Sorption correction of cytokine-mediated immunoreactive changes in the presence of oncogenic endotoxaemia” Soroka Yu.V., Demkiv I.Ya., Lisnychuk N.Ye.
Notice No. 113-2014 “Apoptosis-mediated changes of immune reactivity in the presence of 1.2-dimethylhydrazine-induced colon adenocarcinoma” Soroka Yu.V., Demkiv I.Ya, Lisnychuk N.Ye.
Notice No. 264-2014 “Structural changes of the liver in the presence of chronic neoplastic toxaemia” Volkov K.S., Soroka Yu.V., Demkiv I.Ya, Lisnychuk N.Ye.
Notice No. 263-2014 “Ultrastructural changes of nephron components in the presence of induced oncogenesis” Lisnychuk N.Ye., Volkov K.S., Demkiv I.Ya., Nebesna Z.M., Soroka Yu.V

The staff of the Central Research Laboratory constantly provides practical and methodological assistance to the University’s researchers in completing their doctorate and PhD theses.
The employees the Central Research Laboratory take part in research and training conferences, symposia, etc. regularly.

In 2005-2015, the following fundamental and applied academic research works were carried out in the facilities of the Central Research Laboratory with funding from the Ministry of Health of Ukraine:

1. Clinical-epidemiological and immunological justification of improvement of preventive and therapeutic technologies in cases of influenza and other acute respiratory viral infections.
   Scientific advisers:
   Corresponding Member of the National Academy of Medical Sciences of Ukraine Professor Kovalchuk L.Ya.;
   Corresponding Member of the National Academy of Medical Sciences of Ukraine Professor Andreichyn M.A..
2. Morphofunctional fundamentals of adaptation processes in cardiovascular and digestive systems in cases of arterial hypertension in greater and lesser circulation with different corrective effects.
   Scientific adviser: Professor Hnatiuk M.S..
3. Structural and functional features of the small and large intestines in cases of combined disorders of the organs of pancreatohepatobiliary region.
   Scientific adviser: Associate Professor Lisnychuk N.Ye..
4. Pathogenic impact of polytrauma on internal organs and development of their systemic correction.
   Scientific adviser: Professor Hudyma A.A..
5. Correction of structural and functional changes of the small intestine in cases of combined disorders of the organs of the pancreatohepatobiliary region using biospecific selective immunosorbents and magnetic laser radiation (experimental justification).
   Scientific adviser: Associate Professor Lisnychuk N.Ye..
6. Development of production technique for gritted substrate of conserved pig skin, its experimental and clinical study for treatment of immunological disorderі in adults and children.
   Scientific adviser: Professor Klishch I.M..
7. Development of differentiated methods of correction of hemodynamic and metabolic disorders in the patients with arterial hypertension combined with internal disorders.
   Scientific adviser: Professor Shved M.I..
8. Experimental study of patterns of apoptosis in the presence of severe combined trauma.
   Scientific adviser: Professor Hudyma A.A..
9. Study of morphological state of kidney and large joints in the presence of combined pathologies of the organs of pancreatohepatobiliary region.
   Scientific adviser: Associate Professor Lisnychuk N.Ye..
10. Study of pathogenetic features of multiorgan failure development in the presence of experimental cranioskeletal trauma and search for systemic correction methods by means of cell therapy.
    Scientific adviser: Professor Hudyma A.A..
11. Development of methods for improvement of safety and effectiveness of surgical treatment of the most common surgical abdominal diseases in the presence of polymorbidity.
    Scientific adviser: Professor I.Ya. Dziubanovsky..
12. Structural and metabolic changes of cerebral cortex, kidneys and liver caused by chronic neoplastic intoxication and components of cytostatic therapy and their sorption correction.
    Scientific adviser: Associate Professor Lisnychuk N.Ye..

OPEN PROBLEMS FOR RESEARCHES IN THE CENTRAL RESEARCH LABORATORY:

• the urgency of the issue of pharmacotherapy safety caused necessity for researches on toxic effect of drugs on internal organs as well as searching for effective methods of its correction and prevention;
• a high incidence of oncological diseases substantiates the feasibility of the study of structural and metabolic changes of cerebral cortex, kidneys and liver caused by chronic endogenous neoplastic intoxication and components of cytostatic therapy as well as development of pathogenetically adequate methods for their treatment and prevention;
• the study of impact of oxidative stress and nitric oxide system on the pathogenesis of internal diseases and search for effective methods of their pharmacocorrection.

Laboratory Staff

The Head of the Central Research Laboratory, Senior Researcher, Associate Professor Natalia Lisnychuk
Senior Researcher, PhD. Iryna Demkiv
3rd category Technician Liubov Kalinichuk
Junior Researcher Olha Chykhyra

Address

Central Research Laboratory of I.Ya. Horbachevsky Ternopil National Medical University
19A Hlyboka Street,
Ternopil, 46001,
Ukraine
telephone / fax: +38 (0352) 245002